Examples of applications



Abstract These data were selected by Pavel Jandera, together with Tuulia Hyötyläinen.

These tables show a selection of applications, and their origin.


LevelAdvanced

A printable pdf of all four tables can be downloaded here.

RPLCxRPLC

RPLCxRPLC

Application

1. dimension:
column, eluent

2. dimension:
column, eluent

Reference

Oligostyrenes

C18, methanol

Zirconia, acetonitrile

Gray, M.J., et al J. Chromatogr. A 1041 (2004) 101-110.

Herb extracts, phenolic acids

C18, gradient of acetic acid and methanol

Amino, water/acetonitrile

Kivilompolo et al 
J. Chromatogr. A
,
1145 (2007) 155-164

Plant extract  Stevia glycosides

C18, gradient of water and
acetonitrile

Amino
acetonitrile/H2O

Pól, J.Pol et al. J. Chromatogr. A 1150 (2007) 85-92.

 Corn metabolomics

HS-F5,  Gradient of water and acetonitrile

carbon clad zirconia,  gradient acetonitrile

J. Zhang, et al Anal. Bioanal. Chem. 386 (2006), p. 586

Proteomics

X-terra C18, Gradient of acetonitrile and TFA

SB-Phenyl, Gradient acetonitrile, TFA

C.J. Venkatramani and A. Patel, J. Sep. Sci. 29 (2006), p. 510.

Phenolic antioxidants, flavones in beer

polyethylene glycol silica, gradient of acetonitrile

monolithic C18, ZR-carbon, ammonium acetate + H3PO4 + acetonitrile

F. Cacciola, et al., J. Sep. Sci., 29 (2006), p. 2500.

Indolic acids metabolites in maize

HS-F5 silica, gradient of acetonitrile in phosphate - perchlorate buffer

ZR-carbon, gradient of acetonitrile in water + perchloric acid, 110 oC

D.R. Stall et al., J. Chromatogr. A, 1122 (2006), p. 123.

NPLCxRPLC, RPLCxNPLC

NPLCxRPLC, RPLCxNPLC

Application

1. dimension:
column, eluent

2. dimension:
column, eluent

Reference

Orange essential oil and juice carotenoids

Microbore silica, gradient of  n-hexane and ethyl alcohol

monolithic C18 , gradient of 2-propanol, acetonitrile and water

P. Dugo, et al Anal. Chem. 78 (2006), p. 7743.

 Lemon oil carotenoids

Microbore silica,  Isocratic n-hexane/ ethyl alcohol

monolithic C18, gradient of  water and ACN

P. Dugo, et al J. Chromatogr. Sci. 44 (2006), p. 561.

IEC-RPLC

IEC-RPLC

Application

1. dimension:
column, eluent

2. dimension:
column, eluent

Reference

Tryptic peptides

PO4-zirconia, sodium phosphate gradient

SB-C18, gradient of acetonitrile  and TFA

[D.R. Stoll and P.W. Carr, J. Am. Chem. Soc. 127 (2005), p. 5034.

Proteomics

Polymeric beads bonded with diethylaminoethyl and sulfonic acid groups × Gradient KH2PO4

C18, gradient of acetonitrile  and TFA

K. Wagner, et al J. Chromatogr. A 893 (2000), p. 293

Peptides

Quaternary amine-SAX Stepped guanidine thiocyanate salt

C18,  acetonitrile gradient

L.A. Holland and J.W. Jorgenson, J. Microcolumn Sep. 12 (2000), p. 371.

NPLCxSEC, SECxRPLC, RPLCxSEC

Application

1. dimension:
column, eluent

2. dimension:
column, eluent

Reference

Polymers: degradation product of poly(bisphenol A)carbonate (PC)

Alltech Platinum Silica, Chloroform

HSPgel-RT MB-L/M diethylether × chloroform

L. Coulier, et al, J. Chromatogr. A 1070 (2005), p. 79.

Polystyrene

Hypersil silica, Isocratic THF–hexane

Mixed-C, THF

A. van der Horst and P.J. Schoenmakers, J. Chromatogr. A 1000 (2003), p. 693.

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