Comparison wide-bore with packed and narrow-bore capillary columns

A comparison between the three column types, especially between the wide-bore and the packed column will provide insight into the practical uses and advantages of wide bore columns for particular applications.

  • Efficiency: a small internal diameter and relatively short column length
  • Fast analysis time: high gas velocity: short narrow-bore columns
  • Inertness: Capillary fused-silica columns give good peak shape, suitable for trace analysis
  • Large sample capacity: large column diameter, high amount of stationary phase (film thickness)

Comparison column types 
Column type Efficiency

 

Speed

Capacity
Narrow-bore thin-film            ++      ++ --
Narrow-bore thick-film     + + -
Wide-bore

+/-

+/-

+
Packed, low load - +/- +
Packed, high load -- -- ++

Packed vs. wide-bore column *
Analysis time (min) Efficiency packed column

 

Efficiency wide-bore column

efficiency wide-bore/packed
10             5000      8700 1.7
7.5     4700 7400 1.7
5.0

2800

5800

2.6
* Packed column: 6' x 1/8' SS, 10% SE-30; Wide-bore column:  10 m x 0.53 mm, CP-Sil 5 CB, 5 μm, k = 24 

Packed vs. wide-bore column *
Plate number, Nth Analysis time packed column (min)

Analysis time wide-bore column (min)

Analysis time wide-bore/packed
3000            5.6     3.8 1.5
4000     6.6 4.4 1.5
5000 

10

5.2

1.9
* Packed column: 6' x 1/8' SS, 10% SE-30; Wide-bore column:  10 m x 0.53 mm, CP-Sil 5 CB, 5 μm, k = 24 

Packed vs wide-bore columnsPacked vs wide-bore columns

You really understand separation?


Did you ever try to explain separation to your employees or students? Well, try no more: Lee Polite did it for you in a way which is hard to beat. We will open up one example of his whiteboard class. Click this link to watch the video. To see more, you can register here. Students and teachers can access free for one month.

Discover Chromedia

The links in the center column lead to hundreds of chapters with text, video presentations, visualisations and animations. The chapters on Analytical Chemistry 2.1 (David Harvey), as well as basics LC, GC (Harold McNair, Lee Polite and other experts) and spectroscopy chapters are on the level of colleges, laboratory schools and universities, all 'deeper' clicks lead you to more advanced knowledge on analytical techniques with all the details for in-depth understanding for professional users.
Chromedia is used globally by numerous colleges, universities and industries, our authors are ranked as the finest teachers in the world. So go ahead and discover Chromedia by getting a 30 days subscription, which is free for students and teachers. Chromedia functionalities work best on the Chrome browser.

Chromedia partners:

- Our expert team
- The Analytical Scientist
- American Chemical Society
- Wiley:
- separationsNOW 
- spectroscopyNOW

Register to get full access

For full access to Chromedia: click here for a paid subscription.

Click here to go to the Home-page
Home ->