Introduction to HPLC columns

Abstract The development of HPLC columns has been characterized by decreasing column dimensions and stationary phase particle diameter. These dimensions affect system performance and requirements as well as the final chromatographic quality of the system. Although modern slurry packing techniques produce robust and reproducible columns, their lifetimes still depends on proper handling and storage by the end user. Column longevity can be extended by the selection of the proper stationary phase chemistry and also by the use of guard columns to protect against particulates and column pollution.

KeywordsColumn development, Particle size (dp), Column length (L), Internal diameter (ID), Injection volume, Mobile phase consumption, Analysis time, Pressure drop, Column manufacturing, Column care, Buffers, Storage, Cartridge systems, Guard column, Irreversible binding, Particulates, Efficiency


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