Selecting and optimizing the column
Abstract Column choice is often neglected, mostly due to lack of time or lack of knowledge. It is generally considered too complicated and a non-optimal column is the result. This is a serious pitfall for lab efficiency, since a non-optimal column will lead to time loss, wrong results and mistakes. For column choice, there is no 'silver bullet', no simple answer. One needs to understand more about columns and test methods to make good choices. This investment will pay back. This hapter and related chapters in RPLC will help you.
The resolution, Rs, between any two peaks is an important descriptor of separation quality and is controlled by retention, selectivity and column efficiency. Thus, the resolution is adjusted by changing eluent strength, particle size, modifier type, and many other parameters. This chapter provides some practical guidelines for selection and optimization.
KeywordsColumn choice, Stationary phase, Molecular weight, Hydrophobicity, Ionization, Method optimization, Resolution (Rs), Retention, Selectivity, Efficiency, Reduced plate height, Modifier, Mobile phase, Analysis time, Temperature, Particle size, Column length, Ligand, Mobile phase velocity, Elution strength, Viscosity.
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