Abstract Liquid Solid Chromatography ( LSC ) – also called Normal ( NPLC ) or Straight Phase Chromatography - was one of the first separation techniques. It uses a polar stationary phase and a relatively less polar, mostly organic mobile phase. With the tremendous popularity of the more recent Reversed Phase Liquid Chromatography ( RPLC ), NPLC has been displaced to a certain extent, but is generally underestimated and still attractive. In fact, NPLC is a superior technique for the separation of substances that are chemically closely related, as well for the separation of isomers and diastereomers. NPLC has also found a niche in sample preparation techniques, especially for the separation of complex mixtures within chemical subclasses. This introductory chapter provides the basics and some applications and examples of this technique.


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