High temperature and temperature programmed HPLC

Abstract Authors: Gerd Vanhoenacker and Henk Claessens

The temperature of the HPLC eluent and column has long been an underestimated parameter. Until recently, the best HPLC systems boasted an oven only to thermostat the column in order to improve analytical reproducibility by ensuring a constant temperature. This is extremely surprising given that temperature may influence many important physical parameters in a separation. More recently, however, the HPLC community has recognized temperature’s importance in optimizing separations. Furthermore, temperature has proven to be easier to adjust than the eluent composition.

Performing separations at elevated column temperatures can reduce analysis times, improve column efficiency (plate numbers), reduce peak asymmetry and help optimize retention and selectivity and finally may improve resolution and detectability. With the recent availability of narrow bore columns and the development of high performance ovens, eluent preheaters, and other associated temperature control equipment, LC-analysts have added temperature programming to their arsenal. This chapter describes how to successfully implement HPLC at elevated temperatures.


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