Advantages and limits of HTLC

Abstract This article is written by Sabine Heinisch and Jean-Louis Rocca.

The great potential of High Temperature Liquid Chromatography (HTLC) is discussed on the basis of experimental results obtained at temperatures ranging from 30 to 160°C. The various advantages of high temperatures for the separation of basic compounds are highlighted. These advantages include the great improvement in peak shapes, the possibility to vary the selectivity by changing temperature and the significant decrease in gradient re-equilibration time. A particular attention is drawn to the combination of high temperatures and ultra high pressures in order to evaluate the real increase in separation speed. The current limits accounting for the available columns and instruments are highlighted.

KeywordsHTLC, High Temperature LC, Ultra high pressure LC, pharmaceutical compounds.


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